Among the various fluorescent proteins used in biosensor design, mNeonGreen has been particularly effective due to its brightness, photostability, and rapid maturation. Originally derived from Branchiostoma lanceolatum, mNeonGreen is a monomeric yellow-green fluorescent protein that surpasses GFP in intensity and maturation speed, making it well-suited for many biosensor applications. Its use in voltage indicators has improved signal-to-noise ratios and enhanced the ability to capture fast transient signals, which are critical for studying action potentials and synaptic activity.
Several research groups have incorporated mNeonGreen into advanced GEVIs, where it acts as the fluorescence donor in Förster resonance energy transfer (FRET)-based designs. These biosensors have been applied to monitor neuronal firing, cardiac rhythms, and ion channel function with high temporal and spatial resolution. The ability to visualize voltage changes in single cells and even subcellular compartments has provided deeper insights into the electrical behavior of excitable tissues.
At Allele Biotechnology, we have focused on optimizing fluorescent proteins like mNeonGreen to support research in biosensing and live-cell imaging. By refining protein engineering and expression systems, we aim to contribute to the development of more sensitive and reliable tools for studying cellular electrophysiology.
We hope this discussion provides a useful perspective on the role of mNeonGreen in voltage biosensing. If you are working with fluorescent biosensors and would like to exchange ideas or explore potential applications, we welcome the opportunity to collaborate.
