By introducing specialized sequence edits and optimizing untranslated regions (UTRs), capping structures, and other transcript features, Allele’s scientists have significantly enhanced mRNA stability and translational efficiency. These advancements also allow for robust expression of complex, multi-unit proteins such as some ion channels in mammalian cells without genomic integration or DNA delivery.
In recent experiments, Allele successfully expressed multiple surface proteins such as drug target immune checkpoint protein PD-L1 and neuropeptide G protein-coupled receptors (GPCRs) that failed to express under standard plasmid transfection conditions. Cells transfected with Allele’s optimized mRNA demonstrated strong surface localization and clear signal detection, confirming both efficient expression and proper protein folding. These advances are available through Allele’s mRNA EXPRESS™ platform, which enables researchers to rapidly produce challenging mammalian proteins without DNA transfection.
