One-step Genotyping

 

Allele's propreitary direct lysis buffer releases DNA from mouse tails or blood for genotyping PCR. A one-step reaction using the single buffer system is sufficient for preparing DNA as PCR template; phenol extraction, precipitation, or any further purification is not necessary. The buffer contains a combination of enzyme(s), detergents, and other chemical reagents that will lyse mouse tail tissues or blood cells without destroying DNA. An aliquot is then directly used as template in a genotyping PCR reaction.

 

Allele's One-Step Genotyping Direct PCR system offers a variety of advantages including: 

♦ Simple buffer system: Unique from Allele Biotech Enzymes, this single buffer system is the only product that contains everything for lysis, no need to add protease K or any other components before use. 

♦ Single step reaction: The incubation is to be performed at a convenient 50-55°C temperature for 0.5 hours or longer, and the lysate is ready for PCR. 

♦ Consistent: Works with extremely high success rates and is fully guaranteed if used in combination with Allele-in-One PCR MasterMix system.

♦ Lowering costs: Adds only 40 cents or less to each genotyping sample while saving enormous amounts of material and time from traditional methods.