mNeonGreen

For a span of 20 years, researchers have by and large looked to Aequorea Victoria Green Fluorescent protein (GFP) for their imaging, and tracking needs. Over this time period, the development of GFP has elicited a wide spectrum of colors and optimized versions for a multitude of applications. As imaging moves into the super resolution era, most common fluorescent proteins, including GFP, may not possess certain characteristics beneficial for super resolution applications. With this in mind, Allele now offers a new high performance monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein to date, and is an excellent fusion tag for traditional imaging as well as stochastic single-molecule super resolution imaging. Lastly it is a stellar fluorescence resonance energy transfer (FRET) acceptor for cyan fluorescent proteins.

For more information on mNeonGreen's properties view our publication, "A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum" in the journal Nature Methods.  Click here for Licensing opportunities 

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Fig. 1 mNeonGreen absorbance, excitation, and emission

Fig. 2 Basic Properties mNeonGreen Excitation Max (nm) 506 Emission Max (nm) 517 Quantum Yield 0.8 Extinction Coefficient 116,000 Brightness 92.8 pKa 5.7

Figure 4. Fluorescence imaging of mNeonGreen fusion vectors (from left to right: actin, plasma membrane, microtubules, replication foci).