mTFP1 (monomeric Teal Fluorescent Protein 1) is a bright fluorescent protein engineered for high performance in imaging applications. Derived from the Clavularia coral, mTFP1 emits a distinct teal fluorescence, peaking at 492 nm when excited at 462 nm, making it an excellent choice for multi-color imaging in combination with other fluorescent proteins. Its monomeric nature ensures minimal disruption of tagged protein functions, making it ideal for fusion protein applications.
Key features include:
High Brightness: mTFP1 boasts a high quantum yield (0.85), making it one of the brightest cyan/teal variants available.
Photostability: It is highly resistant to photobleaching, ensuring reliable long-term imaging, even in challenging live-cell microscopy.
Excellent pH Stability: With a pKa of 4.3, mTFP1 maintains its fluorescence intensity in a range of physiological conditions.
Monomeric Structure: Ensures proper folding and minimal perturbation to the protein it is fused with, allowing accurate localization studies.
Ideal for FRET: Its spectral properties make mTFP1 a highly efficient donor in FRET (Förster Resonance Energy Transfer) experiments, especially when paired with yellow or orange acceptors.
mTFP1 is perfect for applications in live-cell imaging, protein localization studies, and multi-color fluorescence microscopy, providing a versatile and reliable tool for cutting-edge research.
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