GFP-nAb

What is GFP-nAb?

Optimized by the research team at Allele Biotechnology, GFP-nAb™ is a highly specific GFP (Green Fluorescent Protein) binding protein derived from camelids. It is characterized by a small barrel shaped structure (13 KDa, 2.5nm X 4.5 nm) and a very high stability (stable up to 70°C, functional within 2M NaCl or 0.5% SDS). Experiments have shown that one molecule of GFP-nAb™ binds one molecule of GFP with a dissociation constant (Kd) in the sub nanomolar range. This makes GFP-nAb™ the ideal candidate for a variety of biological assays, some of which are listed below:

• Immunoprecipitation / CO-IP
• Quantitative analysis
• Chromatin Immunoprecipitation (ChIP)
• Superresolution Imaging

GFP-nAb™ binds a large number of commonly used fluorescent proteins derived from the original Aequorea victoria GFP, including EGFP, Venus, Cerulean, and EBFP2 (click here for a full list). Conveniently, it displays no binding affinity to non-jellyfish-derived fluorescent proteins, including the "mFruits" such as mCherry, or Allele's mNeonGreen, mTFP1, mWasabi, and mMaple fluorescent proteins. The GFP-nAb™ Spin Kit offers a simple, consistent protocol to cleanly pull down GFP fusions. Each GFP-nAb™ Spin Kit comes with all required buffers and spin columns to eliminate inconsistent elutions and allow you to perform multiple immunoprecipiation reactions in a matter of minutes.

Request a Sample

To request a nAb sample Click Here to fill out our sample request form.