GFP-nAb

Scroll Down to Products

What is GFP-nAb?

Optimized by the research team at Allele Biotechnology, GFP-nAb™ is a highly specific GFP (Green Fluorescent Protein) binding protein derived from camelids. It is characterized by a small barrel shaped structure (13 KDa, 2.5nm X 4.5 nm) and a very high stability (stable up to 70°C, functional within 2M NaCl or 0.5% SDS). Experiments have shown that one molecule of GFP-nAb™ binds one molecule of GFP with a dissociation constant (Kd) in the sub nanomolar range. This makes GFP-nAb™ the ideal candidate for a variety of biological assays, some of which are listed below:

  • Immunoprecipitation / CO-IP
  • Quantitative analysis
  • Chromatin Immunoprecipitation (ChIP)
  • Superresolution Imaging

GFP-nAb™ binds a large number of commonly used fluorescent proteins derived from the original Aequorea victoria GFP, including EGFP, Venus, Cerulean, and EBFP2 (click here for a full list). Conveniently, it displays no binding affinity to non-jellyfish-derived fluorescent proteins, including the "mFruits" such as mCherry, or Allele's mNeonGreen, mTFP1, mWasabi, and mMaple fluorescent proteins. The GFP-nAb™ Spin Kit offers a simple, consistent protocol to cleanly pull down GFP fusions. Each GFP-nAb™ Spin Kit comes with all required buffers and spin columns to eliminate inconsistent elutions and allow you to perform multiple immunoprecipiation reactions in a matter of minutes.

gfp-nab-gel.jpg

Complete Pulldown using the GFP-nAb™ Spin Kit.

EGFP-expressing Sf9 (insect) cell lysate contained a total of 16µg of EGFP in total volume of 500µl, determined spectrophotometrically. Following the GFP-nAb™ Spin Kit binding and wash protocols, the protein was eluted in 2 x 50 µl elution buffer (0.2M glycine pH 2.5), pooled, and neutralized with 10µl of 1M Tris base. Equal volumes of lysate input (I), flow-through (FT) after binding to GFP-nAb™ agarose resin, and elution (E) fractions were analyzed by SDS-PAGE followed by Coomassie staining. In this experiment, EGFP was quantitatively removed from the lysate.

Request a Sample

To request a nAb sample Click Here to fill out our sample request form.

  • GFP-nAb Magnetic Agarose Kit (20 Reactions)

    [[20 Rxns,ABP-NAB-GFPXK20]] The GFP-nAb Magnetic Agarose Kit allows for quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ magnetic slurry binds a minimum of 0.5µg of GFP.  This kit contains all the...

    $469.00
    Add to Cart
  • GFP-nAb Kit

    GFP-nAb™ Agarose Spin Kit (20 Reactions)

    [[20 Rxns,ABP-NAB-GFPAK20]] The GFP-nAb™ Agarose Spin Kit allows for quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ agarose slurry binds a minimum of 5µg of GFP.  This kit contains all the...

    $469.00
    Add to Cart
  • GFP-nAb

    GFP-nAb Magnetic Agarose 0.25ml

    [[250µl,ABP-NAB-GFPX025]] GFP-nAb™ Magnetic Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™  magnetic agarose slurry binds a minimum of 4µg of GFP...

    $219.00
    Add to Cart
  • GFP-nAb

    GFP-nAb Magnetic Agarose 0.5ml

    [[500µl,ABP-NAB-GFPX050]] GFP-nAb™ Magnetic Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™  magnetic agarose slurry binds a minimum of 4µg of GFP...

    $399.00
    Add to Cart
  • GFP-nAb

    GFP-nAb Magnetic Agarose 1ml

    [[1ml,ABP-NAB-GFPX100]] GFP-nAb™ Magnetic Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™  magnetic agarose slurry binds a minimum of 4µg of GFP. GFP-nAb™ is the...

    $719.00
    Add to Cart
  • GFP-nAb™ Agarose 0.25ml

    [[250µl,ABP-NAB-GFPA025]] GFP-nAb™ Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ agarose slurry binds a minimum of 5µg of GFP. GFP-nAb™ is the ideal candidate...

    $219.00
    Add to Cart
  • GFP-nAb

    GFP-nAb™ Agarose 0.5ml

    [[500µl,ABP-NAB-GFPA050]] GFP-nAb™ Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ agarose slurry binds a minimum of 5µg of GFP. GFP-nAb™ is the ideal candidate...

    $399.00
    Add to Cart
  • GFP-nAb™ Agarose 1ml

    [[1ml,ABP-NAB-GFPA100]] GFP-nAb™ Agarose resin allows for a quick and convenient pulldown of GFP from solution. 10µl of GFP-nAb™ agarose slurry binds a minimum of 5µg of GFP. GFP-nAb™ is the ideal candidate for a...

    $719.00
    Add to Cart
  • GFP-nAb Purified Protein

    [[25µg,ABP-NAB-GFPAB]] GFP-nAb™ purified protein is a single domain VHH antibody that binds GFP. It is supplied in the amount of 25µg at a 1mg/ml concentration. Components 25µg GFP-nAb™ Purified Protein 1mg/ml...

    $199.00
    Add to Cart
  • nAb Spin Kit Columns and Buffers

    [[20 Reactions,ABP-NAB-SPINK20]] A convenient kit consisting of 20 spin columns and all the required buffers for our nAb line of products.  The nAb Spin Kit Columns and Buffers can be used with our nAb controls or to supplement your existing nAb...

    $99.00
    Add to Cart
  • nAb Agarose Control 0.5ml

    [[500µl,ABP-nAb-ACNTRL5]] Allele Biotech’s Agarose Control resin is convenient and ready to use for preclearing lysate samples before performing an IP reaction using agarose support. The blocked resin is also useful as a negative...

    $49.00
    Add to Cart
  • nAb Magnetic Agarose Control 0.5ml

    [[500µl,ABP-nAb-XCNTRL5]] Allele Biotech’s Magnetic Agarose Control resin is convenient and ready to use for preclearing lysate samples before performing an IP reaction using agarose support. The blocked resin is also useful as a...

    $49.00
    Add to Cart