For a span of 20 years, researchers have by and large looked to Aequorea victoria Green Fluorescent protein (GFP) for their imaging, and tracking needs. Over this time period, the development of GFP has elicited a wide spectrum of colors and optimized versions for a multitude of applications. As imaging moves into the super resolution era, most common fluorescent proteins, including GFP, may not possess certain characteristics beneficial for super resolution applications. With this in mind, Allele now offers a new high performance monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein to date, and is an excellent fusion tag for traditional imaging as well as stochastic single-molecule super resolution imaging. Lastly it is a stellar fluorescence resonance energy transfer (FRET) acceptor for cyan fluorescent proteins. For more information on mNeonGreen's properties view our publication, "A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum" in the journal Nature Methods. mNeonGreen is wholly owned by Allele Biotechnology and subject to US patent 10,221,221. Click here for Licensing opportunities, click here for our Active License List
Allele Biotechnology now has a portal for information on various super resolution imaging techniques (PALM, STORM, SIM, STED), tools, and equipment. To access this super resolution imaging portal click here
Site License and Advanced User license have access to these mNeonGreen fusions and constructs carried by Allele Biotechnology.
|pNCS-mNeonGreen (E.coli Expression)
|Fusions- From: Shaner, N.C. et al. "A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature Methods 2013, ePub March 24.
|mNeonGreen-Endo-14 - RhoB-14
|mNeonGreen-Golgi-7 - GalT-7
|mNeonGreen-Lysosomes-20 - LAMP1-20
|mNeonGreen-MannII-N-10 - MannosidaseII
|mNeonGreen-SiT-N-15 - sialyltransferase
|Yeast Optimized mNeonGreen