Superresolution Imaging - A Nobel Prize Winning Technique - 10/20/2014


In 1873 Ernst Abbe published an equation describing what we commonly refer to as Abbe’s law, defining the practical limits for microscopic resolution. According to Abbe’s law, resolution is limited by the wavelength of visible light; with a minimum value ~400nm the highest achievable resolution would be ~200nm. As astonishing as this level of resolution may be, it isn’t enough for live subcellular research and has often been a limitation for investigators.

This year the Nobel Prize in Chemistry was awarded to three scientists who have developed microscopy techniques circumventing Abbe’s diffraction limit. Eric Betzig, Stefan W. Hell and William E. Moerner received the prize for their pioneering work in the emerging field of superresolution imaging or nanoscopy. Dr. Hell developed stimulated emission depletion (STED) microscopy and Drs. Betzig and Moerner, working independently, developed single-molecule microscopy.

In 2010 Allele Biotechnology made its first foray into field of superresolution microscopy with the release of mClavGR2, a photoconvertible fluorescent protein enabling superresolution techniques. Since then we have released mMaple, a new variant of mClavGR2, and mNeonGreen, a bright, photostable fluorescent protein suitable for single-molecule superresolution imaging. In the future we plan to release newer fluorescent probes and reagents, including nAb nano antibodies, to further support the field of nanoscopy.

To celebrate this momentous occasion for the next two weeks all advanced licenses for mNeonGreen and Photoconvertible fluorescent proteins (mMaple and mClavGR2) will be half off. An advanced license enables you to use these proteins for your research and provides you with unlimited access to our expanding plasmid/fusion library. To take advantage of this offer use the code “SuperRes” when you place your order. Visit our promotions page for more details.