Viral vectors have been used as gene delivery vehicles. Compared to the conventional transfection methods, transduction typically results in much higher gene delivery efficiency, sometimes nearly 100%. This is especially useful for gene delivery into primary cells or stem cells that is recalcitrant to conventional transfection methodologies. However, construction of a viral vector is a laborious process that requires experience and a special lab setup; commercial service in this area has so far been either too expensive or not user-friendly. Therefore, many researchers still use transfection methods.
In 2009, Allele Biotech launched a powerful viral packaging platform for Retrovirus, Lentivirus, and Baculovirus. With our proprietary packaging technologies, Allele-packaged lentiviruses reach 10^8 TU/ml without the need for any concentrating steps. With a concentration step, however, 10^9~10^10 TU/ml can be achieved which is ideal for in vivo research. The Viral Systems are available in 3 sub-groups: 1.) Viral Expression Systems, which provides a series of cloning plasmids, packaging systems and control plasmids; 2.) Custom Packaging Services, which provides the one-stop services from gene synthesis, subcloning to viral packaging; 3.) Pre-Packaged Viruses, expressing fluorescent protein (FP) controls, non-FP controls, reprogramming factors, pluripotency reporters, Cre/loxP, cytokines, and optogenetic factors.
Made-to-Order
Under the Made-to-order program, Allele Biotech will evaluate and accept customers’s requests for packaging cDNA or shRNA in high demand. Special discount will be offered for these accepted projects. Please contact us for detailed information.
Viral Expression Systems
Gryphon™ pCHAC Retrovirus
Retroviruses are considered the easiest and fastest means to deliver genes stably to mammalian cells. Allele has upgraded its previously offered Phoenix retrovirus packaging systemthat provides either an Ecotropic (for murine or rat cells) or an Amphotropic glycoprotein envelope (for most mammalian species, including human) to a more efficient system that routinely results in a titer of 10^8 or higher; and thus, we have discontinued the previously known Phoenix amphotropic and ecotropic cells: a similar Ecotropic and Amphotopic packaging cell line, Phoenix2, originating from different sources are currently available.
- mWasabi; a green fluorescent marker protein.
- Integrated Allele's engineered shRNA expression cassette for the highest efficiency of RNAi (US patents 7,294,504 and 7,422,896).
Sapphire™ Baculovirus
The baculovirus expression system (BVES) provides a high-yield and low-cost platform for expressing functional eukaryotic proteins. Most eukaryotic post-translational modifications, such as signal cleavage, phosphorylation, glycosylation, and disulfide bond formation can occur in baculovirus-infected insect cells. Allele Sapphire™ Baculovirus DNA was specifically designed for efficient recombination with a polyhedrin-based transfer vector by a simple co-transfection step.
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Proprietary baculovirus DNA generates virtually 100% recombinants to eliminate the time-consuming plaque purification step.
- A protein disulfide isomerase gene was inserted at the p10 locus of the virus to ensure proper protein disulfide bond formation.
- The Sapphire™ Baculovirus DNA is compatible with most of the commercial polyhedrin-based transfer vectors.
Custom Services
Lentivirus/Retrovirus for Gene Expression
Allele´s Custom Service Division brought to the market advanced lentivirus and retrovirus packaging services. They are highlighted by a consistent high titer, and large delivery viral counts. Currently, lentivirus packaging requires co-transfecting a number of helper plasmids with typical virus titer yield at 10^6TU (transduction units)/ml. Allele's virus packaging technologies are proprietary and highly efficient, yielding 10^8 to 10^9TU/ml; without any concentrating steps. With a concentration step, however, a titer of 10^9 to 10^10TU/ml can be achieved, which is particularly useful for in vivo uses.
- Unbeatable Prices and bulk discounts
- Sample Infectivity Data and Warranty
- A rich collection of Lentiviral/Retroviral plasmids
Lentivirus/Retrovirus for RNA Interference
Effective delivery of RNAi reagents into cells remains a major barrier for achieving expected gene silencing results. Lentiviral or retroviral vectors provide the highest gene delivery efficiency into difficult-to-transfect cells, i.e., primary cells and in vivo tissues. Combined with the only patented Pol III promoter-driven shRNA or miRNA platform, Allele Biotech provides the most efficient RNAi service:
- Gene-to-Silence RNAi service, from design to virus. 5 viruses cost only $6,899.
- Sequence-to-Virus Full Service, from sequence to virus. Typical cost per shRNA is $1,749 before discounts.
- DNA-to-Virus Packaging Service, from plasmid to virus. As low as $899 with discounts.
Baculovirus & Insect Protein Expression
Allele Biotech/Orbigen Custom Baculovirus Protein Expression Service offers the fastest and most cost-effective way for you to take advantage of the Baculovirus expression technology.
- Up to a 10-fold yield enhancement and fast turnaround time.
- Experienced technical staff with more than 18 years of experience.
- Modularized and customized service.
- Competitive pricing to fit your budget.
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Pre-packaged Viruses
Viruses expressing cDNAs, miRNAs, or shRNAs of broad interest or special purposes are packaged before an order is placed.
Features & Benefits
- Ready to use
- High titer
- Independently validated
Quality Controls & Warranties
These viruses go through our standard quality control processes with validated titer and are ready for use. However, the success of the experiments depends on many factors, some of which are under end user control. Proprietary baculovirus DNA generates virtually 100% recombinants to eliminate the time-consuming plaque purification step.
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BaculoBank
Baculovirus stocks encoding common pharmacological target proteins. For more information please follow link below.
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