Allele-In-One Mouse Tail Direct Lysis Buffer [500 rxns]

Allele-In-One Mouse Tail Direct Lysis Buffer [500 rxns]

$249.00
SKU:
ABP-PP-MT01500
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[[500 rxns,ABP-PP-MT01500]]Allele-In-One Mouse Tail Direct PCR buffer releases DNA from mouse tails for genotyping PCR. A one-step reaction using the single buffer system is sufficient for preparing DNA as PCR template; phenol extraction, precipitation, or any further purification is not necessary. The buffer contains a combination of enzyme(s), detergents, and other chemical reagents that will lyse the mouse tail tissues or other tissues without destroying DNA. An aliquot is then directly used as template in a genotyping PCR reaction.

See Allele-In-One Mouse Tail Lysis Buffer in action: GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice

 

The Mouse Tail Direct PCR system offers a variety of advantages including:

♦ Simple buffer system: Unique from Allele Biotech Enzymes, this single buffer system is the only product that contains everything for lysis, no need to add protease K or any other components before use.

♦ Single step reaction: The incubation is to be performed at a convenient 50-55°C temperature for 0.5 hours or longer, and the lysate is ready for PCR.

♦ Consistent: Works with extremely high success rates and is fully guaranteed if used in combination with Allele-in-One PCR MasterMix system.

♦ Lowering costs: Adds only 40 cents or less to each genotyping sample while saving enormous amounts of material and time from traditional methods.

 

Click here to download or print TDS.

Our clients love our buffers!

“I have been using the Allele mouse tail lysis buffer for a while and have observed an improvement in the quality of genotype bands, making them more visible and defined. This is particularly crucial for some transgenic mice, as the DNA extraction step becomes essential in reducing unreliable results. The relatively low frequency of false negatives and inconsistent performance among different experiments highlight the importance of this extraction process when using a good reagent.”

Camila Marques De Freria, Ph.D. - Tuszynski's lab - UCSD Neurosciences

Shipping:

Domestic orders can now be shipped at room temperature overnight, saving on shipping weight and costs! Store the lysis buffer immediately upon arrival. Lysis buffer can last for 2 weeks stored at 4 degrees Celsius, for long term storage store the buffers at -20 degrees Celsius.

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