mScarlet3 has the record high brightness as a red fluorescent protein, and in cells, more than fivefold brighter than mCherry. With a pKa=4.2, mScarlet3 has strong acid resistance, particularly beneficial for endomembrane studies. On the other end of the pH range, this fluorescent protein retains 50% of fluorescence for over 40 minutes at pH13.5. mScarlet3 displays monoexponentially fluorescence decay with long lifetimes of 4.0 ns, facilitating possible application as donors in FRET with fluorescence lifetime imaging (FLIM).
Based on comparison studies, mScarlet3 is the best FRET acceptor of YFPs. As a matter of fact, it will make an outstanding FRET pair with Allele’s other popular fluorescent protein in the green/yellow spectrum—mNeonGreen!
Deliver mScarlet3 mRNA EXPRESS™ to your various cell types and observe how they express the bright red protein with higher expression and higher efficiency than traditional methods.
mScarlet3 is covered by patent application WO2022253948A1.
Product Information:
1ug of total mRNA per tube, 10 tubes total, dilute according to your needs, a total volume of 10ng of mRNA was delivered to the cells pictured above.
10ug of mRNA per order.
Immediately upon arrival store this reagent at -80°C.
FAQ
Q: Do I have to combine any reagents before delivering this to my cells?
A: No! Allele's mRNA EXPRESS™ is formulated so it can be delivered to your cells directly. All you have to do is thaw the vial and you are ready to go!
Q: How quickly will my cells express the desired protein?
A: Expression can be seen in as quick as 2 hours post transfection!
Q: Why should I choose mRNA EXPRESS™ over my current method of transfection?
A: This product is one of a kind! You are able to skip the tedious steps of traditional transfection while achieve even higher expression and transfection efficiency!
