Allele-In-One Cell Direct PCR Kit [500 rxns]

$537.00
SKU:
ABP-CDP500
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Allele-In-One Cell Direct Lysis Buffer releases DNA from cells for genotyping PCR. A one-step reaction using the single buffer system is sufficient for preparing DNA as PCR template; phenol extraction, precipitation, or any further purification is not necessary. The buffer contains a combination of enzyme(s), detergents, and other chemical reagents that will lyse the cells without destroying DNA. An aliquot is then directly used as template in a genotyping PCR reaction.

 

The Cell Direct Lysis Buffer system offers a variety of advantages including:

♦ Simple buffer system: Unique from Allele Biotech Enzymes, this single buffer system is the only product that contains everything for lysis, no need to add protease K or any other components before use.

♦ Single step reaction: The incubation is to be performed at a convenient 50-55°C temperature for 0.5 hours or longer, and the lysate is ready for PCR.

♦ Consistent: Works with extremely high success rates and is fully guaranteed if used in combination with Allele-in-One PCR MasterMix system.

♦ Lowering costs: Adds only 40 cents or less to each genotyping sample while saving enormous amounts of material and time from traditional methods.

 

Click here to download or print TDS.

 

Shipping:

Domestic orders can now be shipped at room temperature overnight, saving on shipping weight and costs! Store the lysis buffer immediately upon arrival. Lysis buffer can last for 2 weeks stored at 4 degrees Celsius, for long term storage store the buffers at -20 degrees Celsius.